The Microbial Production of Polyhydroxyalkanoates from Waste Polystyrene Fragments Attained Using Oxidative Degradation
Autor: | Iza Radecka, Adam A. Marek, Surila Darbar, Brian Johnston, Daniel J. Keddie, Magdalena Zięba, Barbara Mendrek, David Hill, Marek Kowalczuk, Wanda Sikorska, Grazyna Adamus, Vassilka Ivanova Ilieva, Emo Chiellini, Marta Musioł |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
polyhydroxyalkanoates (PHAs)
Polymers and Plastics Cupriavidus necator chemistry.chemical_element Biomass 02 engineering and technology 010501 environmental sciences recycling 01 natural sciences Bioplastic Polyhydroxyalkanoates Article lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry prodegraded fermentation 0105 earth and related environmental sciences mass spectrometry chemistry.chemical_classification biology General Chemistry Polymer polystyrene (PS) 021001 nanoscience & nanotechnology biology.organism_classification Pulp and paper industry bioplastics chemistry Fermentation Polystyrene 0210 nano-technology Carbon |
Zdroj: | Polymers Polymers, Vol 10, Iss 9, p 957 (2018) Volume 10 Issue 9 |
ISSN: | 2073-4360 |
Popis: | Excessive levels of plastic waste in our oceans and landfills indicate that there is an abundance of potential carbon sources with huge economic value being neglected. These waste plastics, through biological fermentation, could offer alternatives to traditional petrol-based plastics. Polyhydroxyalkanoates (PHAs) are a group of plastics produced by some strains of bacteria that could be part of a new generation of polyester materials that are biodegradable, biocompatible, and, most importantly, non-toxic if discarded. This study introduces the use of prodegraded high impact and general polystyrene (PS0). Polystyrene is commonly used in disposable cutlery, CD cases, trays, and packaging. Despite these applications, some forms of polystyrene PS remain financially and environmentally expensive to send to landfills. The prodegraded PS0 waste plastics used were broken down at varied high temperatures while exposed to ozone. These variables produced PS flakes (PS1&ndash 3) and a powder (PS4) with individual acid numbers. Consequently, after fermentation, different PHAs and amounts of biomass were produced. The bacterial strain, Cupriavidus necator H16, was selected for this study due to its well-documented genetic profile, stability, robustness, and ability to produce PHAs at relatively low temperatures. The accumulation of PHAs varied from 39% for prodegraded PS0 in nitrogen rich media to 48% (w/w) of dry biomass with the treated PS. The polymers extracted from biomass were analyzed using nuclear magnetic resonance (NMR) and electrospray ionization tandem mass spectrometry (ESI-MS/MS) to assess their molecular structure and properties. In conclusion, the PS0&ndash 3 specimens were shown to be the most promising carbon sources for PHA biosynthesis with 3-hydroxybutyrate and up to 12 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units generated. |
Databáze: | OpenAIRE |
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