Antibody enhances killing of Tritrichomonas foetus by the alternative bovine complement pathway
| Autor: | M K Aydintug, R W Leid, P R Widders |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Complement Pathway
Alternative Immunology Antibodies Protozoan Hemolysis Microbiology Complement factor B Classical complement pathway Animals Bovine serum albumin Complement Activation Egtazic Acid Tritrichomonas biology Adenine Complement System Proteins biology.organism_classification Virology Molecular biology Complement system Infectious Diseases Alternative complement pathway biology.protein Cattle Parasitology Tritrichomonas foetus Antibody Complement Factor B Research Article |
| Zdroj: | Infection and Immunity. 58:944-948 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.58.4.944-948.1990 |
| Popis: | The role of bovine antibody and complement in host defense against Tritrichomonas foetus was measured by using an assay of trichomonad viability based on protozoal uptake of tritiated adenine. Moderate killing was measured in the absence of antibody only with high concentrations of complement-preserved hypogammaglobulinemic bovine serum. However, very low concentrations of hyperimmune serum promoted significant enhancement (P less than 0.05) of killing by complement. Heat inactivation of complement (56 degrees C for 30 min) eliminated antibody-dependent and -independent killing. Similarly, depletion of bovine factor B in serum by heat treatment (50 degrees C for 45 min) abolished antibody-dependent and -independent killing. However, selective inactivation of the classical complement pathway with magnesium ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid did not affect antibody-dependent or -independent killing by complement. These findings demonstrate antibody enhancement of complement-mediated killing of T. foetus by the alternative pathway of bovine complement. |
| Databáze: | OpenAIRE |
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