Sequence Variation in the E2-Binding Domain of HPV16 and Biological Function Evaluation in Tunisian Cervical Cancers
| Autor: | Lotfi Kochbati, Ridha Oueslati, Mongi Maalej, Mohamed Badis Chanoufi, Saloua Kahla, Samia Hammami |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Tunisia Article Subject Papillomavirus E7 Proteins Uterine Cervical Neoplasms Repressor lcsh:Medicine Biology DNA-binding protein General Biochemistry Genetics and Molecular Biology law.invention Protein structure law Genetic variation Humans Peptide sequence Gene Polymerase chain reaction Aged Genetics Human papillomavirus 16 General Immunology and Microbiology Papillomavirus Infections lcsh:R Genetic Variation Oncogene Proteins Viral General Medicine Middle Aged Molecular biology Protein Structure Tertiary DNA-Binding Proteins Repressor Proteins Female Research Article Binding domain |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | HPV16 E2 variants have different effects on the transcriptional activity of the LCR. In this study, we examined the nucleotide and amino acid sequence variation within the HPV16 E2 gene and to correlate with disease progression. E2 gene disruption was detected by PCR amplification of the entire E2 gene using a single set of primers. Nucleotide variations were analyzed by bidirectional sequencing. mRNA expression patterns of E6 and E7 gene transcripts were evaluated by a reverse transcriptase-PCR method (RT-PCR). The detection of intact E2 genes was significantly higher among controls than cases (81.8% versus 37.5%, resp.,P<0.05). Among the E subgroup, variation at position 3684 C>A results in the amino acid substitution T310K and was more common among the E2 undisrupted cases (7/9; 77.7%), compared to controls (2/9; 22.2%). In addition, specific sequence variations identified in the E2 ORF at positions 3684 C>A were associated with increased viral oncogenes E6-E7 production. Besides HPV16 E2 disruption, the 3684 C>A variation within undisrupted E2 genes could be involved in an alternative mechanism for deregulating the expression of the HPV16 E6 and E7 oncogenes and appears to be a major factor contributing to the development of cervical cancer in Tunisian women. |
| Databáze: | OpenAIRE |
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