An optical pickup enzyme-linked immunosorbent assay (ELISA) with a microfluidic disk
| Autor: | Makoto Yoshinaga, Eiichi Tamiya, Hiroyuki Yoshikawa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Chromatography Materials science Co2 laser biology General Chemical Engineering 010401 analytical chemistry Microfluidics 02 engineering and technology General Chemistry Optical pickup 021001 nanoscience & nanotechnology 01 natural sciences Primary and secondary antibodies Reflectivity 0104 chemical sciences biology.protein 0210 nano-technology Laser beams |
| Zdroj: | RSC Advances. 8:14510-14514 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c8ra01149d |
| Popis: | We evaluated optical pickup ELISA with an original microfluidic disk that contains eight radially arranged channels, which enable semi-automatic sample loading and washing. This disk-shaped chip composed of acrylic plates was fabricated by CO2 laser machining and capture antibodies were immobilized in the channels. After the immunoreaction with antigens and enzyme-linked secondary antibodies, an enzyme-catalyzed nanoaggregation of o-phenylenediamine was detected by measuring the reflectivity change of a laser beam focused in the channel. The assay of C-reactive protein (CRP) was successfully performed in a short amount of time (approximately 20 min from CRP loading). The limit of detection was determined to be 2 ng mL−1, which is more sensitive as compared with conventional ELISA using microplates. |
| Databáze: | OpenAIRE |
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