Control of morphogenetic cell movements in the early zebrafish myotome
| Autor: | Peter D. Currie, Sharon L. Amacher, David F. Daggett, Carmen R. Domingo |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Notochord
Adaxial Biology cap Article 03 medical and health sciences 0302 clinical medicine Myotome Cell Movement medicine Animals Myocyte Somite Molecular Biology Zebrafish Actin Body Patterning 030304 developmental biology Cuboidal Cell Muscle Cells 0303 health sciences Gene Expression Regulation Developmental Apical constriction Cell Biology Anatomy Zebrafish Proteins biology.organism_classification Cell biology medicine.anatomical_structure Somites Mutation Muscle Carrier Proteins 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. 309:169-179 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2007.06.008 |
| Popis: | As the vertebrate myotome is generated, myogenic precursor cells undergo extensive and coordinated movements as they differentiate into properly positioned embryonic muscle fibers. In the zebrafish, the “adaxial” cells adjacent to the notochord are the first muscle precursors to be specified. After initially differentiating into slow-twitch myosin-expressing muscle fibers, these cells have been shown to undergo a remarkable radial migration through the lateral somite, to populate the superficial layer of slow-twitch muscle of the mature myotome. Here we characterize an earlier set of adaxial cell behaviors; the transition from a roughly 4 × 5 array of cuboidal cells to a 1 × 20 stack of elongated cells, prior to the migration event. We find that adaxial cells display a highly stereotypical series of behaviors as they undergo this rearrangement. Furthermore, we show that the actin regulatory molecule, Cap1, is specifically expressed in adaxial cells and is required for the progression of these behaviors. The requirement of Cap1 for a cellular apical constriction step is reminiscent of similar requirements of Cap during apical constriction in Drosophila development, suggesting a conservation of gene function for a cell biological event critical to many developmental processes. |
| Databáze: | OpenAIRE |
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