Characterization of a pluripotent stem cell-derived matrix with powerful osteoregenerative capabilities
| Autor: | Fei Liu, Suzanne Zeitouni, Daniel Tahan, Michael Cesarek, Qingguo Zhao, Bret H. Clough, Carl A. Gregory, W. Brian Saunders, Simin Pan, Ulf Krause, Lauren K. Dobson, Roland Kaunas, Andrew W. Haskell, Christopher Kung, Mayra Garcia, Eoin P. McNeill |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Collagen Type XII Pluripotent Stem Cells Science General Physics and Astronomy Bone Morphogenetic Protein 2 Collagen Type VI Matrix (biology) Bone morphogenetic protein Bone morphogenetic protein 2 General Biochemistry Genetics and Molecular Biology Bone tissue engineering Article Craniofacial Abnormalities 03 medical and health sciences Mice 0302 clinical medicine Collagen VI Osteogenesis Animals Humans Induced pluripotent stem cell lcsh:Science Cells Cultured Cell Proliferation Multidisciplinary Molecular medicine Tissue Engineering Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Chemistry In vitro Cell biology Tissues Induced pluripotent stem cells 030104 developmental biology Regenerative medicine lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-16646-2 |
| Popis: | Approximately 10% of fractures will not heal without intervention. Current treatments can be marginally effective, costly, and some have adverse effects. A safe and manufacturable mimic of anabolic bone is the primary goal of bone engineering, but achieving this is challenging. Mesenchymal stem cells (MSCs), are excellent candidates for engineering bone, but lack reproducibility due to donor source and culture methodology. The need for a bioactive attachment substrate also hinders progress. Herein, we describe a highly osteogenic MSC line generated from induced pluripotent stem cells that generates high yields of an osteogenic cell-matrix (ihOCM) in vitro. In mice, the intrinsic osteogenic activity of ihOCM surpasses bone morphogenic protein 2 (BMP2) driving healing of calvarial defects in 4 weeks by a mechanism mediated in part by collagen VI and XII. We propose that ihOCM may represent an effective replacement for autograft and BMP products used commonly in bone tissue engineering. Production of a safe and manufacturable material to mimic anabolic bone for tissue engineering has been hard to achieve to date. Here the authors use a mesenchymal stem cell line generated from induced pluripotent stem cells to produce osteogenic cell-matrix, displaying significant healing properties in mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|