| Popis: |
The study was conducted in the Tissue Culture Laboratory of the Field Crops Department of Agricultural Faculty of the Ege University in 2020-2021. Bulbs of Muscari neglectum collected from nature were used as genetic material in the study. These bulbs were cultured in nutrient media containing MS (Control) and MS+ TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) 1.0; 2.0; 3.0; 4.0 for the regeneration. When the results were estimated; the highest means were obtained in MS+4.0 mg/l TDZ medium in terms of shoot number (8.2), shoot length (4.9 cm). MS+1.0 mg/l BAP medium had the highest means for root number (7.5), root length (3.3 cm). The shoots developed from these media were sub cultured in media containing MS+IBA for rooting. The media containing MS+2.0 mg/l IBA was found to be the highest in terms of plant number (8.3), plant height (8.7 cm) and root number (6.0). Muscari neglectum's bulbs have regeneration potential under in vitro conditions. The possibilities of using this plant as an ornamental or medicinal plant can be increased by using this technology |
