Flow cytometric evaluation of peripheral blood and bone marrow and fine-needle aspirate samples from multiple sites in dogs with multicentric lymphoma
| Autor: | Ingo Nolte, Alexa E Joetzke, Reinhard Mischke, D. Simon, N. Eberle |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphoma Biopsy Fine-Needle Spleen Statistics Nonparametric Flow cytometry Dogs Bone Marrow Cytology Biopsy medicine Animals Dog Diseases Lymph node Analysis of Variance General Veterinary medicine.diagnostic_test business.industry General Medicine Flow Cytometry medicine.disease medicine.anatomical_structure Liver Bone marrow business Fine-needle aspirate |
| Zdroj: | American Journal of Veterinary Research. 73:884-893 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.73.6.884 |
| Popis: | Objective—To determine whether the extent of disease in dogs with lymphoma can be assessed via flow cytometry and to evaluate the suitability of fine-needle aspirates from the liver and spleen of dogs for flow cytometric examination. Animals—44 dogs with multicentric B-cell (n = 35) or T-cell lymphoma (9) and 5 healthy control dogs. Procedures—Peripheral blood and bone marrow samples and fine-needle aspirates of lymph node, liver, and spleen were examined via flow cytometry. Logarithmically transformed T-cell–to–B-cell percentage ratio (log[T:B]) values were calculated. Thresholds defined by use of log(T:B) values of samples from control dogs were used to determine extranodal lymphoma involvement in lymphoma-affected dogs; results were compared with cytologic findings. Results—12 of 245 (5%) samples (9 liver, 1 spleen, and 2 bone marrow) had insufficient cellularity for flow cytometric evaluation. Mean log(T:B) values of samples from dogs with B-cell lymphoma were significantly lower than those of samples from the same site in dogs with T-cell lymphoma and in control dogs. In dogs with T-cell lymphoma, the log(T:B) of lymph node, bone marrow, and spleen samples was significantly higher than in control dogs. Of 165 samples assessed for extranodal lymphoma involvement, 116 (70%) tested positive via flow cytometric analysis; results agreed with cytologic findings in 133 of 161 (83%) samples evaluated via both methods. Conclusions and Clinical Relevance—Results suggested that flow cytometry may aid in detection of extranodal lymphoma involvement in dogs, but further research is needed. Most fine-needle aspirates of liver and spleen were suitable for flow cytometric evaluation. |
| Databáze: | OpenAIRE |
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