Histamine release from human basophils by the insect allergen Chi t I
| Autor: | Gertraud Mazur, Xaver Baur, Barbara Jarosch |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Hypersensitivity Immediate Allergy Immunology Radioimmunoassay Immunoglobulin E medicine.disease_cause Histamine Release Chironomidae chemistry.chemical_compound Allergen Radioallergosorbent Test medicine Immunology and Allergy Animals Humans Sensitization Aged Skin Tests biology medicine.diagnostic_test Radioallergosorbent test Antibodies Monoclonal General Medicine Allergens Middle Aged medicine.disease Basophils medicine.anatomical_structure chemistry biology.protein Antibody Histamine |
| Zdroj: | International archives of allergy and applied immunology. 91(4) |
| ISSN: | 0020-5915 |
| Popis: | Hemoglobins of the Diptera species Chironomus thummi thummi (Chi t I) are potent inhalant allergens. Chi t I-specific histamine release was measured by a new radioimmunoassay in whole human blood taken from 20 sensitized patients, 11 exposed nonsensitized probands, and 11 nonexposed controls. The sensitized patients, who all had positive skin tests and radioallergosorbent test results with Chi t I, showed a significantly higher histamine release than the two other groups. However, within the patient group, the percentage of released histamine did not correlate with the intensity of the skin test response or the concentration of Chi t I-specific IgE antibodies. Our results demonstrate that this method is a sensitive and specific in vitro test for evaluation of IgE-mediated sensitization. |
| Databáze: | OpenAIRE |
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