Detection and Characterization of Circulating Tumor Cells in Patients with Merkel Cell Carcinoma
| Autor: | Julie Waldispühl-Geigl, Gerold Schuler, Ingrid Moll, Sven Peine, Ellen Heitzer, Klaus Pantel, Sabine Riethdorf, Michael R. Speicher, Sonja Bergmann, Lucie Heinzerling, Nicole Fischer, Oliver Mauermann, Cornelia Coith, Lina Hildebrandt, Gerhard Schön |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Clinical Biochemistry Merkel cell polyomavirus Cell Count Kaplan-Meier Estimate B7-H1 Antigen 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Internal medicine medicine Carcinoma Humans Aged Proportional Hazards Models biology Proportional hazards model Merkel cell carcinoma business.industry Incidence (epidemiology) Biochemistry (medical) Neoplastic Cells Circulating Prognosis medicine.disease biology.organism_classification Immune checkpoint Carcinoma Merkel Cell 030104 developmental biology 030220 oncology & carcinogenesis DNA Viral Female Skin cancer business |
| Zdroj: | Clinical Chemistry. 65:462-472 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | BACKGROUND Merkel cell carcinoma (MCC) is a rare, aggressive skin cancer with increasing incidence and high mortality rates. MCC has recently become the subject of immune checkpoint therapy, but reliable biomarkers for estimating prognosis, risk stratification, and prediction of response are missing. METHODS Circulating tumor cells (CTCs) were detected in peripheral blood from patients with MCC by use of the CellSearch® system. Moreover, CTCs of selected cases were characterized for Merkel cell polyomavirus (MCPyV), chromosomal aberrations, and programed death ligand 1 (PD-L1) production. RESULTS Fifty-one patients were tested at first blood draw (baseline), and 16 patients had 2 or 3 consecutive measurements to detect CTCs. At baseline, ≥1 CTC (range, 1–790), >1, or ≥5 CTCs/7.5 mL were detected in 21 (41%), 17 (33%), and 6 (12%) patients, respectively. After a median follow-up of 21.1 months for 50 patients, detection of CTCs correlated with overall survival (≥1, P = 0.030; >1, P < 0.020; and ≥5 CTCs/7.5 mL, P < 0.0001). In multivariate Cox regression analysis, the detection of ≥5 CTCs/7.5 mL adjusted to age and sex compared to that of CONCLUSIONS The presence of CTCs is a prognostic factor of impaired clinical outcome, with the potential to monitor the progression of the disease in real time. Molecular characterization of CTCs might provide new insights into the biology of MCC. |
| Databáze: | OpenAIRE |
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