Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans
| Autor: | A. Djeballi, Pierre-Louis Blaiseau, Marc-Henri Lebrun, Catherine Gerlinger, Marie-Josée Daboussi, D. Parisot, Yves Brygoo, I. Bouvier, M. Cassan |
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| Rok vydání: | 1989 |
| Předmět: |
Aspergillus
biology Genes Fungal Mutant Nucleic Acid Hybridization General Medicine Fungi imperfecti Nitrate reductase biology.organism_classification Aspergillus nidulans Microbiology Transformation (genetics) Transformation Genetic Nitrate Reductases Mutation Fusarium oxysporum Chlorates Genetics DNA Fungal Gene Plasmids |
| Zdroj: | Current Genetics. 15:453-456 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00376803 |
| Popis: | A gene transfer system originally developed for Fusarium oxysporum has been applied to seven species of filamentous fungi of agricultural and industrial importance. This transformation system relies on the selection of mutants deficient in nitrate reductase by positive screening. Such mutants were recovered easily in all the fungi tested--without mutagenic treatments--through their resistance to chlorate. They were transformed by a plasmid vector (pAN301) carrying the Aspergillus nidulans wild-type gene (niaD). Transformation frequencies ranged from one to ten transformants/micrograms plasmid DNA. The general properties of the transformants were analyzed. Most of them are mitotically stable, and the integration of the vector into the host genome frequently occurred in a tandem fashion. |
| Databáze: | OpenAIRE |
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