Separation of IgE from IgG Subclasses Using Staphylococcal Protein A
| Autor: | H C Cox, J J Adcock, H Zola, L G Garland |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Immunology
Staphylococcal protein General Medicine Immunoglobulin E Biology Chromatography Affinity Rats chemistry.chemical_compound medicine.anatomical_structure chemistry Immunoglobulin G Protein A/G medicine biology.protein Animals Humans Immunology and Allergy Antibody Binding site Staphylococcal Protein A Protein A Histamine Sensitization |
| Zdroj: | International Archives of Allergy and Immunology. 56:123-127 |
| ISSN: | 1423-0097 1018-2438 |
| Popis: | When human serum was passed through a column of insolubilised protein A, IgE was not removed, but IgG subclasses 1, 2 and 4 were retained. Protein A did not show a similar selectivity in its interaction with rat immunoglobulins. The separation of human IgE from the bulk of the IgG was used to examine the effect of IgG on IgE-mediated sensitization of lung tissue for histamine release. Removal of IgG led to a significant increase in histamine release, suggesting that homocytotropic IgG competes with IgE for binding sites on mast cells. |
| Databáze: | OpenAIRE |
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