Diagnosis of spontaneous bacterial peritonitis in children using leukocyte esterase reagent strips and granulocyte elastase immunoassay
| Autor: | Maha M Allam, Olfat Hendy, Mohsen Hassan Hussin, Haidy Mohammed Zakaria, Sara Mohammed Eltaras, El-Sayed Ibrahem Salama, Alif Allam |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Ascitic fluid
Original Paper medicine.medical_specialty Granulocyte Elastase Reagent strip Hepatology medicine.diagnostic_test business.industry Dipstick granulocyte elastase medicine.disease Gastroenterology spontaneous bacterial peritonitis Leukocyte esterase Spontaneous bacterial peritonitis Internal medicine Immunoassay Ascites medicine leukocyte esterase cardiovascular diseases medicine.symptom business circulatory and respiratory physiology |
| Zdroj: | Clinical and Experimental Hepatology |
| ISSN: | 2392-1099 |
| Popis: | Aim of the study We aimed to assess the utility and rapidity of granulocyte elastase (GE) latex immunoassay and leukocyte esterase (LE) reagent strips for the diagnosis of spontaneous bacterial peritonitis (SBP) in hepatic children with ascites. Material and methods This study included 80 ascitic fluid (AF) samples from 45 patients with chronic liver diseases. They were divided into 2 groups (SBP and non-SBP groups). White blood cells > 500 cell/mm3 or polymorphonuclear leukocytes > 250 cell/mm3 were considered as a positive result for the diagnosis of SBP. The AF obtained at bedside was immediately tested with an LE enzyme reagent strip, and another sample was aliquoted for measurement of serum GE. Results On doing LE dipstick strips, all the non-SBP group gave no coloration with LE strips while 62% of the SBP group gave coloration. LE strips had accuracy of 86.25% in differentiating SBP and non-SBP at a cut-off value of 1 (color grade 1). The diagnostic performance of GE in differentiating SBP and non-SBP was assessed and showed accuracy of 70% for a cutoff value of 123.5 ng/ml. Conclusions Application of LE reagent strips is a bedside, rapid, easy-to-use, and inexpensive method for diagnosis of SBP. It has an accuracy of 86.25% in differentiating SBP and non-SBP, which is higher than more complicated and delayed methods such as GE latex immunoassay, which has an accuracy of 70%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|