DNA hypermethylation and clinicopathological features in breast cancer: the Western New York Exposures and Breast Cancer (WEB) Study
| Autor: | Janet Winston, Jo L. Freudenheim, Meng-Hua Tao, Bin Xie, Christine B. Ambrosone, Stephen B. Edge, Dominica Vito, Amy E. Millen, Shiva Krishnan, Peter G. Shields, Catalin Marian, Maurizio Trevisan, Jing Nie |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Oncology Cancer Research medicine.medical_specialty Pathology Receptors Retinoic Acid New York Estrogen receptor Breast Neoplasms medicine.disease_cause Article Breast cancer Internal medicine Progesterone receptor Humans Medicine Neoplasm Metastasis Cyclin-Dependent Kinase Inhibitor p16 Aged Reverse Transcriptase Polymerase Chain Reaction business.industry Case-control study Cancer DNA Methylation Middle Aged Cadherins medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic Postmenopause Case-Control Studies DNA methylation Breast disease Receptors Progesterone business Carcinogenesis |
| Zdroj: | Breast Cancer Research and Treatment. 114:559-568 |
| ISSN: | 1573-7217 0167-6806 |
| Popis: | Aberrant DNA hypermethylation of gene promoter regions has been increasingly recognized as a common molecular alteration in carcinogenesis. We evaluated the association between major clinicopathological features and hypermethylation of genes in tumors among 803 incidence breast cancer cases from a large population-based case-control study conducted in Western New York State. DNA samples were isolated from archive paraffin embedded tumor tissue and were analyzed for hypermethylation status of the E-cadherin, p16, and RAR-beta(2) genes using real time methylation-specific polymerase chain reaction. The frequencies of hypermethylation were 20.0% for E-cadherin, 25.9% for p16, and 27.5% for RAR-beta(2) genes. For postmenopausal women, hypermethylation of E-cadherin tended to be more likely in progesterone receptor (PR) negative than in PR-positive tumors (odds ratio (OR), 1.41; 95% confidence interval (CI), 0.91-2.18). Hypermethylation of p16 tended to be more frequent among estrogen receptor (ER) negative cases than ER-positive cases (OR, 1.51; 95% CI, 1.01-2.32). Hypermethylation of RAR-beta(2) gene was inversely associated with histological and nuclear grade of breast cancer. |
| Databáze: | OpenAIRE |
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