Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis
| Autor: | Robert Kranc, Elena Belonovskaya, Oxana Lukivskaya, Vyacheslav Buko, Rostislav S. Stoika, Natalia Sybirna, Olena Kanyuka, Olga Zhuk, Elena Naruta |
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| Rok vydání: | 2015 |
| Předmět: |
Liver Cirrhosis
medicine.medical_specialty Cirrhosis Connective tissue Thioacetamide Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Fibrosis Internal medicine Genes Regulator Animals Medicine General Pharmacology Toxicology and Pharmaceutics Mice Knockout Tumor Necrosis Factor-alpha business.industry General Medicine medicine.disease Immunohistochemistry Hepatic stellate cell activation Securin Hydroxyproline medicine.anatomical_structure Endocrinology Liver chemistry Hepatic stellate cell Tumor necrosis factor alpha business Hepatic fibrosis Biomarkers |
| Zdroj: | Life Sciences. 132:34-40 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2015.04.010 |
| Popis: | Aims Pituitary tumor-transforming gene (PTTG) is involved in multiple cellular pathways. We studied the development of liver fibrosis induced by thioacetamide (TAA) in knockout (PTTG −/−) and wildtype (PTTG +/+) mice. Main methods Liver fibrosis in PTTG +/+ and PTTG −/− mice was induced by escalating dose TAA treatment (50–400 mg/kg, i.p.) for 12 weeks and assessed by histochemistry, immunohistochemistry, liver hydroxyproline, serum fibrosis markers and fibrosis-related mRNA expression by real-time PCR determination. Key findings Both PTTG +/+ and PTTG −/− mice treated with TAA developed signs of fibrosis and inflammatory cell infiltration. However, histological signs of bridging fibrosis and connective tissue square morphometry were significantly attenuated in mice lacking PTTG. α-SMA immunohistochemistry revealed that hepatic stellate cell activation was markedly reduced in PTTG −/− mice compared to wildtype controls. Hepatic hydroxyproline levels were significantly lower in fibrotic PTTG −/− group. The serum TNFα and hepatic TNFα mRNA expression were significantly lower in fibrotic PTTG −/− animals, as well as hepatic TGFβ and VEGF mRNA levels compared to TAA-treated wildtype controls. Serum hyaluronate and TGFβ levels were markedly elevated in fibrotic mice of both genotypes, but were not altered by the absence of PTTG. Significance TAA-induced fibrosis development is significantly ameliorated in PTTG −/− mice. These animals demonstrated diminished stellate cell activation, suppressed circulating serum markers of inflammation, fibrogenesis and angiogenesis. The presented findings suggest that PTTG is functionally required for hepatic fibrosis progression in an animal model of chronic liver injury. PTTG can be considered as a new important target for prevention and treatment of liver fibrosis/cirrhosis. |
| Databáze: | OpenAIRE |
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