Evidence for direct effect of magnetic fields on neurite outgrowth

Autor:	S. G. Benane, Carl F. Blackman, Dennis E. House
Rok vydání:	1993
Předmět:	Electromagnetic field Field (physics) Neurite Chemistry Anatomy Biochemistry PC12 Cells Magnetic field Rats Magnetics Nerve growth factor Electricity Cell culture Electric field Genetics Biophysics Neurites Animals Nerve Growth Factors Molecular Biology Cell culture assays Biotechnology
Zdroj:	FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 7(9)
ISSN:	0892-6638
Popis:	Electric fields can cause changes in cell responses both in vitro and in vivo. Alternating magnetic fields have been proposed to act through the electric fields induced in the conducting medium surrounding the cells. We have used a simple exposure system to test the relative contribution of magnetic fields compared to induced electric fields in a standard PC-12 cell culture assay, in which cells respond to nerve growth factor by producing neurites. This response to stimulation by nerve growth factor is inhibited by sinusoidal, 50-Hz magnetic fields at field strengths below 10 microT (100 mG). A standard procedure to distinguish magnetic- vs. electric-field effects demonstrates that the induced electric field is not involved. Additional work is necessary to identify the critical reaction site (or sites), and to establish the molecular mechanisms responsible for these results.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d96a4adc211d04f260b6dc41b87f2cf1 https://pubmed.ncbi.nlm.nih.gov/8330687 Zobrazit plný text záznamu Plný text