Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS
| Autor: | Pratap Singhasivanon, Jeremy Farrar, Nicholas P. J. Day, Benjamas Kamanikom, Niklas Lindegardh, Tran Tinh Hien, Warunee Hanpithakpong, Nicholas J. White |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Drug
Bioanalysis media_common.quotation_subject Clinical Biochemistry Pharmacology Severe influenza Plasma volume Tandem mass spectrometry Antiviral Agents Article Analytical Chemistry Plasma Zanamivir Tandem Mass Spectrometry Influenza Human medicine Humans General Pharmacology Toxicology and Pharmaceutics media_common Chromatography Chemistry General Medicine Reference Standards Medical Laboratory Technology Hilic ms ms Anti influenza drug Chromatography Liquid medicine.drug |
| Zdroj: | Bioanalysis. 3(2) |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Background: Parenteral zanamivir is a promising drug for the treatment of severe influenza. However, quantification of this polar drug in biological matrices has traditionally been difficult and the methods developed have been relatively insensitive. Results: A high-throughput bioanalytical method for the analysis of zanamivir in human plasma using SPE in the 96-well plate format and LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 50 µl of plasma and covers a large working range from 1–50, 000 ng/ml with a LOD of 0.50 ng/ml. Conclusion: This new LC–MS/MS assay is more sensitive than previous methods despite using a small plasma volume sample. It is particularly suitable for clinical studies on both parenteral and inhaled zanamivir. |
| Databáze: | OpenAIRE |
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