Creation of Bifunctional Indicating Complex Based on Nanodiamonds and Extracellular Oxidases of Luminous Fungus Neonothopanus nambi
| Autor: | N. O. Ronzhin, Vladimir S. Bondar, O. A. Mogilnaya, K. S. Artemenko |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biophysics 02 engineering and technology Biochemistry Catalysis Nanodiamonds Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Extracellular Phenol Glucose oxidase Bifunctional Neonothopanus nambi chemistry.chemical_classification biology Basidiomycota General Chemistry General Medicine 021001 nanoscience & nanotechnology Enzymes Immobilized Combinatorial chemistry 030104 developmental biology Enzyme chemistry biology.protein 0210 nano-technology Oxidoreductases Peroxidase |
| Zdroj: | Doklady. Biochemistry and biophysics. 480(1) |
| ISSN: | 1608-3091 |
| Popis: | A bifunctional indicating complex was created by immobilization of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MNDs) synthesized by detonation. It was found that the enzymes firmly adsorb onto MND particles and exhibit their catalytic activity. Model in vitro experiments showed that the created MND-enzymes complex is suitable for repeated use for analyte (glucose and phenol) testing and retains its activity after storage at 4°C in deionized water for 1 month. The data obtained offer the prospects for developing a new class of reusable multifunctional indicating and diagnostic test systems on the basis of MNDs and higher fungal enzymes for medical and ecological analytics. |
| Databáze: | OpenAIRE |
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