Preventing Surgical Site Infections Using a Natural, Biodegradable, Antibacterial Coating on Surgical Sutures

Autor: Stefanie Krajewski, Jürgen Geis-Gerstorfer, Hans Peter Wendel, Ingrid Müller, Christian Schlensak, Tobias Weindl, Ann-Kristin Uhde, Jochen Reinbold
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Totarol
Pharmaceutical Science
02 engineering and technology
medicine.disease_cause
Bacterial Adhesion
Analytical Chemistry
chemistry.chemical_compound
Mice
Suture (anatomy)
Coating
Coated Materials
Biocompatible
Polylactic Acid-Polyglycolic Acid Copolymer
Drug Discovery
Drug Carriers
totarol
suture
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
PLGA
Chemistry (miscellaneous)
Staphylococcus aureus
Drug delivery
surgical site infection
antibacterial coating
biodegradable
Molecular Medicine
Diterpenes
0210 nano-technology
medicine.medical_specialty
Cell Survival
Surface Properties
030106 microbiology
engineering.material
Article
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
medicine
Animals
Surgical Wound Infection
Agar diffusion test
Physical and Theoretical Chemistry
Sutures
business.industry
Organic Chemistry
Biofilm
Fibroblasts
Surgery
Drug Liberation
chemistry
Biofilms
Abietanes
engineering
Microscopy
Electron
Scanning
business
Zdroj: Molecules, Vol 22, Iss 9, p 1570 (2017)
Molecules; Volume 22; Issue 9; Pages: 1570
Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
ISSN: 1420-3049
Popis: Surgical site infections (SSIs) are one of the most common nosocomial infections, which can result in serious complications after surgical interventions. Foreign materials such as implants or surgical sutures are optimal surfaces for the adherence of bacteria and subsequent colonization and biofilm formation. Due to a significant increase in antibiotic-resistant bacterial strains, naturally occurring agents exhibiting antibacterial properties have great potential in prophylactic therapies. The aim of this study was to develop a coating for surgical sutures consisting of the antibacterial substance totarol, a naturally occurring diterpenoid isolated from Podocarpus totara in combination with poly(lactide-co-glycolide acid) (PLGA) as a biodegradable drug delivery system. Hence, non-absorbable monofilament and multifilament sutures were coated with solutions containing different amounts and ratios of totarol and PLGA, resulting in a smooth, crystalline coating. Using an agar diffusion test (ADT), it became evident that the PLGA/totarol-coated sutures inhibited the growth of Staphylococcus aureus over a period of 15 days. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the coated sutures were not cytotoxic to murine fibroblasts. Overall, the data indicates that our innovative, biodegradable suture coating has the potential to reduce the risk of SSIs and postoperative biofilm-formation on suture material without adverse effects on tissue.
Databáze: OpenAIRE
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