Hemostatic and toxinological diversities in venom of Micrurus tener tener, Micrurus fulvius fulvius and Micrurus isozonus coral snakes
| Autor: | Amalid Estrella, Ana María Salazar, Elda E. Sánchez, Carlos Ibarra, María E. Girón, Zoila Carvajal, Luis F. Navarrete, Alexis Rodríguez-Acosta, Jeilyn Vivas, Belsy Guerrero, Amparo Gil |
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| Rok vydání: | 2010 |
| Předmět: |
food.ingredient
Platelet Aggregation Venom Toxicology complex mixtures Hemostatics Article Microbiology Lethal Dose 50 Mice Plasma food Fibrinolytic Agents Species Specificity Animals Humans Micrurus Elapidae Envenomation Blood Coagulation Coral snake Elapid Venoms Mice Inbred BALB C biology Ophidia biology.organism_classification Immunology Electrophoresis Polyacrylamide Gel Female Fibrinolytic agent |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 58(1) |
| ISSN: | 1879-3150 |
| Popis: | The coral snake Micrurus tener tener (Mtt) from the Elapidae family inhabits the southwestern United States and produces severe cases of envenomations. Although the majority of Mtt venom components are neurotoxins and phospholipase A2s, this study demonstrated, by SDS-PAGE and molecular exclusion chromatography (MEC), that these venoms also contain high-molecular-weight proteins between 50 and 150 kDa that target the hemostatic system. The biological aspects of other Micrurus venoms were also studied, such as the LD50s of Micrurus isozonus (from 0.52 to 0.61 mg/kg). A pool from these venoms presented a LD50 of 0.57 mg/kg, Micrurus f. fulvius (Mff) and Mtt had LD50s of 0.32 and 0.78 mg/kg, respectively. These venoms contained fibrino(geno)lytic activity, they inhibited platelet aggregation, as well as factor Xa and/or plasmin-like activities. M. isozonus venoms from different Venezuelan geographical regions inhibited ADP-induced platelet aggregation (from 50 to 68%). Micrurus tener tener venom from the United States was the most active with a 95.2% inhibitory effect. This venom showed thrombin-like activity on fibrinogen and human plasma. Fractions of Mtt showed fibrino(geno)lytic activity and inhibition on plasmin amidolytic activity. Several fractions degraded the fibrinogen Aα chains, and fractions F2 and F7 completely degraded both fibrinogen Aα and Bβ chains. To our knowledge, this is the first report on thrombin-like and fibrino(geno)lytic activity and plasmin or factor Xa inhibitors described in Micrurus venoms. Further purification and characterization of these Micrurus venom components could be of therapeutic use in the treatment of hemostatic disorders. |
| Databáze: | OpenAIRE |
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