Intestinal osmoregulatory mechanisms differ in Mediterranean and Atlantic European sea bass: A focus on hypersalinity
| Autor: | Quanquan Cao, Catherine Lorin-Nebel, Eva Blondeau-Bidet |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Gills
0106 biological sciences Mediterranean climate Salinity Environmental Engineering food.ingredient Zoology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Bass (fish) Mediterranean sea food Osmoregulation Hypersalinity Animals Environmental Chemistry Seawater Dicentrarchus labrax 14. Life underwater Sea bass Waste Management and Disposal 030304 developmental biology Water transport 0303 health sciences biology Intraspecific comparison biology.organism_classification Pollution Intestine Intestines Bass Dicentrarchus Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Science Of The Total Environment (0048-9697) (Elsevier BV), 2022-01, Vol. 804, P. 150208 (14p.) |
| Popis: | European sea bass (Dicentrarchus labrax) migrate towards habitats where salinity can reach levels over 60‰, notably in Mediterranean lagoons. D. labrax are genetically subdivided in Atlantic and Mediterranean lineages and have evolved in slightly different salinities. We compared Atlantic and West-Mediterranean populations regarding their capacity to tolerate hypersalinity with a focus on the involvement of the intestine in solute-driven water reabsorption. Fish were analyzed following a two-week transfer from seawater (SW, 36‰) to either SW or hypersaline water (HW, 55‰). Differences among lineages were observed in posterior intestines of fish maintained in SW regarding NKA activities and mRNA expressions of nkaα1a, aqp8b, aqp1a and aqp1b with systematic higher levels in Mediterranean sea bass. High salinity transfer triggered similar responses in both lineages but at different magnitudes which may indicate slight different physiological strategies between lineages. High salinity transfer did not significantly affect the phenotypic traits measured in the anterior intestine. In the posterior intestine however, the size of enterocytes and NKA activity were higher in HW compared to SW. In this tissue, nka-α1a, nkcc2, aqp8ab and aqp8aa mRNA levels were higher in HW compared to SW as well as relative protein expression of AQP8ab. For aqp1a, 1b, 8aa and 8b, an opposite trend was observed. The sub-apical localization of AQP8ab in enterocytes suggests its role in transepithelial water reabsorption. Strong apical NKCC2/NCC staining indicates an increased Na+ and Cl- reuptake by enterocytes which could contribute to solute-coupled water reuptake in cells where AQP8ab is expressed. |
| Databáze: | OpenAIRE |
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