Proteomics unveil corticoid-induced S100A11 shuttling in keratinocyte differentiation

Autor: Xavier Dezitter, Bernadette Masselot, Hervé Drobecq, Thierry Idziorek, Denise Mendy, Fatma Hammoudi, Pierre Formstecher, Jean-Christophe Deloulme, Nicolas Belverge
Přispěvatelé: Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Transduction du signal : signalisation calcium, phosphorylation et inflammation, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Collaboration, Andrieux, Annie, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Keratinocytes
Proteomics
Apoptosis
Mitochondrion
Biochemistry
Dexamethasone
MESH: Cell Compartmentation
Protein purification
Cells
Cultured
0303 health sciences
MESH: Middle Aged
MESH: Proteomics
030302 biochemistry & molecular biology
S100 Proteins
Cell Differentiation
Middle Aged
MESH: Keratinocytes
Cell biology
medicine.anatomical_structure
MESH: Calcium
MESH: Dexamethasone
Female
Keratinocyte
MESH: S100 Proteins
MESH: Cells
Cultured
Adult
MESH: Cell Differentiation
Biophysics
Active Transport
Cell Nucleus
chemistry.chemical_element
MESH: Active Transport
Cell Nucleus
Calcium
Biology
03 medical and health sciences
Organelle
medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Molecular Biology
Glucocorticoids
030304 developmental biology
Calcium metabolism
MESH: Humans
MESH: Apoptosis
Cell Membrane
MESH: Adult
Cell Biology
Cell Compartmentation
chemistry
Cytoplasm
MESH: Glucocorticoids
MESH: Female
MESH: Cell Membrane
Zdroj: Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications, 2007, 360 (3), pp.627-32. ⟨10.1016/j.bbrc.2007.06.113⟩
Biochemical and Biophysical Research Communications, Elsevier, 2007, 360 (3), pp.627-32. ⟨10.1016/j.bbrc.2007.06.113⟩
ISSN: 0006-291X
1090-2104
DOI: 10.1016/j.bbrc.2007.06.113⟩
Popis: International audience; Unlike classical protein extraction techniques, proteomic mapping using a selective subcellular extraction kit revealed S100A11 as a new member of the S100 protein family modulated by glucocorticoids in keratinocytes. Glucocorticoids (GC)-induced S100A11 redistribution in the "organelles and membranes" compartment. Microscopic examination indicated that glucocorticoids specifically routed cytoplasmic S100A11 toward perinuclear compartment. Calcium, a key component of skin terminal differentiation, directed S100A11 to the plasma membrane as previously reported. When calcium was added to glucocorticoids, minor change was observed at the proteomic level while confocal microscopy revealed a rapid and dramatic translocation of S100A11 toward plasma membrane. This effect was accompanied by strong nuclear condensation, loss of mitochondrial potential and DNA content, and increased high molecular weight S100A11 immunoreactivity, suggesting corticoids accelerate calcium-induced terminal differentiation. Finally, our results suggest GC-induced S100A11 relocalization could be a key step in both keratinocyte homeostasis and glucocorticoids side effects in human epidermis.
Databáze: OpenAIRE
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