Caspofungin in Combination with Amphotericin B against Candida parapsilosis
| Autor: | Elisabetta Spreghini, Serena Tomassetti, Francesco Barchiesi, Daniele Giannini, Giorgio Scalise |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Antifungal Agents Echinocandin medicine.drug_class Antibiotics Colony Count Microbial Microbial Sensitivity Tests Biology Pharmacology Kidney Candida parapsilosis Peptides Cyclic Microbiology Echinocandins Lipopeptides Mice chemistry.chemical_compound Caspofungin Amphotericin B medicine Animals Experimental Therapeutics Pharmacology (medical) Candida Broth microdilution Candidiasis biology.organism_classification medicine.disease Drug Combinations Infectious Diseases chemistry Systemic candidiasis Antagonism medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 51:941-945 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00880-06 |
| Popis: | C andida parapsilosis has emerged as an important nosocomial pathogen. In the present study, a checkerboard broth microdilution method was performed to investigate the in vitro activities of caspofungin (CAS) in combination with amphotericin B (AMB) against three clinical isolates of C. parapsilosis . Although there was a significant reduction of the MIC of one or both drugs used in combination, an indifferent interaction (fractional inhibitory concentration index greater than 0.50 and less than or equal to 4.0) was observed in 100% of cases. This finding was confirmed by killing curve studies. By a disk diffusion assay, the halo diameters produced by antifungal agents in combination were often significantly greater than those produced by each drug alone. Antagonism was never observed. In a murine model of systemic candidiasis, CAS at either 0.25 or 1 mg/kg/day combined with AMB at 1 mg/kg/day was significantly more effective than each single drug at reducing the colony counts in kidneys. Higher doses of the echinocandin (i.e., 5 and 10 mg/kg/day) combined with the polyene did not show any advantage over CAS alone. Overall, our study showed a positive interaction of CAS and AMB against C. parapsilosis . |
| Databáze: | OpenAIRE |
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