Baicalin inhibits Escherichia coli isolates in bovine mastitic milk and reduces antimicrobial resistance
| Autor: | M. Jiang, W.M. Zhang, Y.R. Luo, T. Liang, Q.Y. Zhao, S.Z. Qing, F.W. Yuan, X.C. Liang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbial Sensitivity Tests Quinolones medicine.disease_cause beta-Lactamases law.invention Microbiology 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance law Drug Resistance Bacterial Genetics medicine Escherichia coli Animals Mastitis Bovine Polymerase chain reaction Escherichia coli Infections Flavonoids biology Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences Antimicrobial Alkaline Phosphatase 040201 dairy & animal science Enzyme assay In vitro Anti-Bacterial Agents 030104 developmental biology Milk biology.protein Alkaline phosphatase Animal Science and Zoology Cattle Female Baicalin Food Science Plasmids |
| Zdroj: | Journal of dairy science. 101(3) |
| ISSN: | 1525-3198 |
| Popis: | In this study, we aimed to evaluate the inhibitory effect of baicalin on Escherichia coli in vitro and the effects of baicalin treatment on antimicrobial resistance of the E. coli isolates. Through isolation, purification, and identification, a total of 56 E. coli strains were isolated from 341 mastitic milk samples. The study of inhibition effect of baicalin on the E. coli strains in vitro was focused on permeability and morphology of the isolates using an alkaline phosphatase kit and scanning electron microscopy. Furthermore, the resistance spectrum of the isolates to the common antimicrobial agents was tested at sub-minimum inhibitory concentrations of baicalin by the agar dilution method. Extended-spectrum β-lactamase and plasmid-mediated quinolone resistance genes were amplified by PCR before and after incubation with baicalin. The results revealed that baicalin has certain inhibitory effects on the isolates in vitro. The alkaline phosphatase enzyme activity was significantly increased from 1.246 to 2.377 U/100 mL, and the surface of E. coli was concave and shriveled. Analysis of the resistance spectrum and PCR amplification showed that, after administration with baicalin, the sensitivity of most strains to the selected antimicrobial agents was enhanced. Strikingly, the drug-resistant genes from 71.43% (40/56) of these isolates were found to have drug-resistant genes to different extents. Altogether, the current study confirmed both the inhibitory effect on Escherichia coli in vitro and the reduction of antimicrobial resistance by baicalin. This is the first comprehensive study to report on baicalin, a traditional Chinese medicine that acts on E. coli isolated from the mastitic milk samples. |
| Databáze: | OpenAIRE |
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