Impairment of methionine sulfoxide reductase during UV irradiation and photoaging
| Autor: | Marielle Moreau, Emmanuelle Noblesse, Bertrand Friguet, Isabelle Petropoulos, Mylène Juan, Cédric R. Picot, Carine Nizard |
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| Přispěvatelé: | Human Computer Technology Laboratory (HCTLab), Universidad Autonoma de Madrid (UAM) |
| Rok vydání: | 2007 |
| Předmět: |
Aging
Ultraviolet Rays Protein Carbonylation Photoaging medicine.disease_cause Biochemistry 03 medical and health sciences chemistry.chemical_compound Endocrinology Genetics medicine Humans Molecular Biology ComputingMilieux_MISCELLANEOUS Skin 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species Microscopy Confocal Methionine sulfoxide 030302 biochemistry & molecular biology Cell Biology medicine.disease Skin Aging Amino acid Enzyme Activation Oxidative Stress chemistry Methionine Sulfoxide Reductases Medicine Methionine sulfoxide reductase Oxidoreductases Reactive Oxygen Species Oxidative stress MSRA |
| Zdroj: | Experimental Gerontology Experimental Gerontology, Elsevier, 2007, 42 (9), pp.859. ⟨10.1016/j.exger.2007.02.006⟩ |
| ISSN: | 0531-5565 |
| DOI: | 10.1016/j.exger.2007.02.006 |
| Popis: | During chronic UV irradiation, which is part of the skin aging process, proteins are damaged by reactive oxygen species resulting in the accumulation of oxidatively modified protein. UV irradiation generates irreversible oxidation of the side chains of certain amino acids resulting in the formation of carbonyl groups on proteins. Nevertheless, certain amino acid oxidation products such as methionine sulfoxide can be reversed back to their reduced form within proteins by specific repair enzymes, the methionine sulfoxide reductases A and B. Using quantitative confocal microscopy, the amount of methionine sulfoxide reductase A was found significantly lower in sun-exposed skin as compared to sun-protected skin. Due to the importance of the methionine sulfoxide reductase system in the maintenance of protein structure and function during aging and conditions of oxidative stress, the fate of this system was investigated after UVA irradiation of human normal keratinocytes. When keratinocytes are exposed to 15 J/cm(2) UVA, methionine sulfoxide reductase activity and content are decreased, indicating that the methionine sulfoxide reductase system is a sensitive target for UV-induced inactivation. |
| Databáze: | OpenAIRE |
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