Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii
| Autor: | Vincent L. Chiang, Robert M. Kelly, Jack P. Wang, Michael W. W. Adams, Piyum A. Khatibi, Amanda M. Williams-Rhaesa, Christopher T. Straub, Jonathan M. Conway, Ilona Peszlen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Bioalcohols Science General Physics and Astronomy Lignocellulosic biomass Biomass macromolecular substances 02 engineering and technology Lignin complex mixtures Article General Biochemistry Genetics and Molecular Biology Applied microbiology Metabolic engineering Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound Polysaccharides Food science Cellulose lcsh:Science Caldicellulosiruptor bescii Clostridiales Multidisciplinary Ethanol biology fungi technology industry and agriculture food and beverages General Chemistry Industrial microbiology Plants Genetically Modified 021001 nanoscience & nanotechnology biology.organism_classification Populus 030104 developmental biology Metabolic Engineering chemistry Fermentation lcsh:Q 0210 nano-technology |
| Zdroj: | Nature Communications Nature Communications, Vol 10, Iss 1, Pp 1-6 (2019) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-11376-6 |
| Popis: | Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. Metabolizing lignocellulosic feedstocks to industrial chemicals by microorganisms requires surmounting the recalcitrance caused by lignin. Here, the authors pair transgenic lignin modified poplar lines with engineered Caldicellusiruptor bescii to achieve biomass solubilization and ethanol conversion without pretreatment. |
| Databáze: | OpenAIRE |
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