Novel mechanism of macrolide resistance in Streptococcus pneumoniae
| Autor: | Virginia D. Shortridge, S.Ken Tanaka, J. Beyer, Nancy C. Ramer, Robert K. Flamm |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Antibiotics Erythromycin Microbial Sensitivity Tests Biology medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Bacterial Proteins law Streptococcus pneumoniae medicine Polymerase chain reaction Antibacterial agent DNA Primers tRNA Methyltransferases Clindamycin Membrane Transport Proteins Drug Resistance Microbial General Medicine biology.organism_classification Streptococcaceae Anti-Bacterial Agents Infectious Diseases Genes Bacterial Biological Assay Carboxylic Ester Hydrolases Ribosomes Bacteria medicine.drug MSRA |
| Zdroj: | Diagnostic microbiology and infectious disease. 26(2) |
| ISSN: | 0732-8893 |
| Popis: | The mechanism of macrolide resistance was examined in 73 clinical isolates of Streptococcus pneumoniae . Two distinct resistance phenotypes were observed: high-level macrolides-lincosamides-streptogramin B (MLS) resistance and low-level macrolide resistance with lincosamide susceptibility. High-level MLS resistance was associated with the presence of ermAM . Strains with the low-level resistant phenotype (novel) were negative for ermA, ermC, ermAM, ereA, ereB and msrA by polymerase chain reaction (PCR) amplification with genespecific primers. Ribosomes isolated from novel strains bound the same amount of [ 14 C]-erythromycin as ribosomes from sensitive strains. These novel strains also did not inactivate the macrolide. The novel mechanism was found in 41% of the erythromycin resistant S. pneumoniae examined. |
| Databáze: | OpenAIRE |
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