SWATH-MS quantitative proteomic investigation of nitrogen starvation in Arabidopsis reveals new aspects of plant nitrogen stress responses
| Autor: | Ying Zhou, Shi Xiao, Feng Yang, Clive Lo, Jianhua Zhang, Tao Song, Yuan Tian, Li-Juan Xie, Moxian Chen, Wai-Lung Chan, Fu-Yuan Zhu |
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| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0106 biological sciences 0301 basic medicine Proteome Nitrogen Arabidopsis Biophysics Protein metabolism chemistry.chemical_element Photosynthesis 01 natural sciences Biochemistry Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Stress Physiological Membrane fluidity chemistry.chemical_classification Nitrates biology Arabidopsis Proteins Chemistry Lipid metabolism biology.organism_classification Adaptation Physiological Amino acid Phenotype 030104 developmental biology Seedlings 010606 plant biology & botany |
| Zdroj: | Journal of Proteomics. 187:161-170 |
| ISSN: | 1874-3919 |
| Popis: | Nitrogen is an essential macronutrient for plant growth and crop productivity. The aim of this work was to further investigate the molecular events during plant adaptation to nitrogen stress. Here, we present a SWATH-MS (Sequential window acquisition of all theoretical mass spectra)-based quantitative approach to detect proteome changes in Arabidopsis seedlings following nitrogen starvation. In total, 736 proteins of diverse functions were determined to show significant abundance changes between nitrogen-supplied and nitrogen-starved Arabidopsis seedlings. Functional categorization revealed the involvement of nitrogen stress-responsive proteins in biological processes including amino acid and protein metabolism, photosynthesis, lipid metabolism and glucosinolate metabolism. Subsequent phospholipid profiling of Arabidopsis seedlings showed changes in phospholipid composition that may enhance membrane fluidity as a response to nitrogen starvation. Moreover, an Arabidopsis grf6 T-DNA insertion mutant was found to have a nitrogen stress-sensitive phenotype. GRF6 is a 14-3-3 protein with elevated abundance upon nitrogen starvation and it may function as a positive regulator during nitrogen stress adaptation. |
| Databáze: | OpenAIRE |
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