Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
| Autor: | Weronika Grzybek, Michal Liput, Ewa Poławska, Agata Nawrocka, Agnieszka Roszczyk, Aneta Jończy, Paweł Urbański, Magdalena Ogłuszka, Agnieszka Szostak, Chandra S. Pareek, Adam Lepczyński, Mariusz Pierzchała, Kamila P Liput |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Proteomics 0301 basic medicine medicine.medical_specialty SFA Proteome Linoleic acid Carbohydrate metabolism omega-6 PUFA Diet High-Fat Article Linoleic Acid Mice 03 medical and health sciences chemistry.chemical_compound Polyunsaturated fat GSTP1 0302 clinical medicine Fatty Acids Omega-6 Internal medicine medicine Animals TX341-641 ARG1 Gel electrophoresis chemistry.chemical_classification Nutrition and Dietetics Nutrition. Foods and food supply MALDI TOF/TOF Body Weight alpha-Linolenic Acid food and beverages nutriproteomics 2DE 030104 developmental biology Endocrinology Liver chemistry 030220 oncology & carcinogenesis Fatty Acids Unsaturated omega-3 PUFA lipids (amino acids peptides and proteins) HFD Food Science Polyunsaturated fatty acid |
| Zdroj: | Nutrients, Vol 13, Iss 1678, p 1678 (2021) Nutrients Volume 13 Issue 5 |
| ISSN: | 2072-6643 |
| Popis: | The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n–6) to α-linolenic acid (ALA, C18:3n–3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3–10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB ↑: ALDH1L1, UniProtKB—Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets. |
| Databáze: | OpenAIRE |
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