Role of near-infrared fluorescence imaging in the resection of metastatic lymph nodes in an optimized orthotopic animal model of HNSCC
| Autor: | Amandine Hurbin, M. Henry, Ihab Atallah, Jean-Luc Coll, Christian Righini, R. Quatre, E. Reyt, C. Milet |
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| Přispěvatelé: | Biologie des organismes marins et écosystèmes (BOME), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Department of Otolaryngology and Head and Neck Surgery, University Hospital of Grenoble, BP 217, 38043, Grenoble Cedex 09, France, Université Joseph Fourier - Grenoble 1 (UJF), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), University Department of Otorhinolaryngology, Grenoble Hospital, Grenoble, France., Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF) |
| Rok vydání: | 2015 |
| Předmět: |
Near-Infrared Fluorescence Imaging
Pathology medicine.medical_specialty Fluorescence-lifetime imaging microscopy [SDV]Life Sciences [q-bio] Mice Nude Fluorescence Mice RAFT-c(RGD)4 medicine Metastatic adenopathy Animals Lymph node business.industry Squamous Cell Carcinoma of Head and Neck Near-infrared fluorescence imaging-guided surgery αvβ3 integrin Head and neck cancer Metastasectomy Head and neck squamous cell carcinoma medicine.disease Head and neck squamous-cell carcinoma 3. Good health Disease Models Animal medicine.anatomical_structure Surgery Computer-Assisted Otorhinolaryngology Cervical lymph nodes Head and Neck Neoplasms Lymphatic Metastasis Carcinoma Squamous Cell Lymph Node Excision Female Surgery Lymph business Neoplasm Transplantation |
| Zdroj: | European Annals of Otorhinolaryngology, Head and Neck Diseases European Annals of Otorhinolaryngology, Head and Neck Diseases, 2015, 132 (6), pp.337-342. ⟨10.1016/j.anorl.2015.08.022⟩ European Annals of Otorhinolaryngology, Head and Neck Diseases, Elsevier Masson, 2015, 132 (6), pp.337-342. ⟨10.1016/j.anorl.2015.08.022⟩ |
| ISSN: | 1879-7296 1879-730X |
| DOI: | 10.1016/j.anorl.2015.08.022 |
| Popis: | International audience; Keywords: Near-infrared fluorescence imaging-guided surgery Head and neck squamous cell carcinoma Metastatic adenopathy vˇ3 integrin RAFT-c(RGD)4 a b s t r a c t Objectives: To study the role of near-infrared fluorescence imaging in the detection and resection of metastatic cervical lymph nodes in head and neck cancer. Materials and methods: CAL33 head and neck cancer cells of human origin were implanted in the oral cavity of nude mice. The mice were followed up after tumor resection to detect the development of lymph node metastases. A specific fluorescent tracer for ˛vˇ3 integrin expressed by CAL33 cells was injected intravenously in the surviving mice between the second and the fourth month following tumor resection. A near-infrared fluorescence-imaging camera was used to detect tracer uptake in metastatic cervical lymph nodes, to guide of lymph-node resection for histological analysis. Results: Lymph node metastases were observed in 42.8% of surviving mice between the second and the fourth month following orthotopic tumor resection. Near-infrared fluorescence imaging provided real-time intraoperative detection of clinical and subclinical lymph node metastases. These results were confirmed histologically. Conclusion: Near infrared fluorescence imaging provides real-time contrast between normal and malignant tissue, allowing intraoperative detection of metastatic lymph nodes. This preclinical stage is essential before testing the technique in humans. |
| Databáze: | OpenAIRE |
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