Protein tyrosine kinase expression during the estrous cycle and carcinogenesis of the mammary gland
| Autor: | Martin Flueck, Anne-Catherine Andres, Andrew Ziemiecki, Valentin Djonov, Gisela Zuercher |
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| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
medicine.medical_specialty animal structures Molecular Sequence Data Mammary gland Gene Expression Apoptosis Biology medicine.disease_cause Mice Mammary Glands Animal Estrus Pregnancy Lactation Internal medicine Cell Adhesion medicine Animals Humans Neoplastic transformation Amino Acid Sequence RNA Messenger Estrous cycle Neovascularization Pathologic Sequence Homology Amino Acid Mammary Neoplasms Experimental Receptor Protein-Tyrosine Kinases Protein-Tyrosine Kinases Extracellular Matrix Hematopoiesis Cell biology Mice Inbred C57BL medicine.anatomical_structure Endocrinology Oncology Mammary Epithelium Hybridization Genetic Female Signal transduction Carcinogenesis Sequence Alignment Tyrosine kinase Cell Division Signal Transduction |
| Zdroj: | International Journal of Cancer. 63:288-296 |
| ISSN: | 1097-0215 0020-7136 |
| Popis: | The susceptibility of the mammary epithelium to neoplastic transformation is linked to the exposure to estrogen during the estrous cycle. The effects of the estrous cycle on the mouse mammary gland have been investigated by analyzing 3 H-thymidine incorporation, milk protein gene expression and DNA fragmentation. We found that the mammary epithelium undergoes limited proliferation, differentiation and apoptosis in a cycle-dependent manner. The estrous-responsive regulators of the mammary epithelium are unknown ; however, considering the integral role of protein tyrosine kinases (PTKs) in the control of normal and malignant development, members of this family of enzymes are likely candidates for such regulatory molecules. Using a RT-PCR-based cloning strategy, we have undertaken a survey of PTKs expressed in the mammary gland at defined stages of development, with special emphasis on the estrous cycle. We identified 21 known and 4 novel PTKs. Their expression was analyzed throughout mammary gland development and in mammary neoplasias using a transgenic mouse model for invasive and non-invasive carcinogenesis. Most of the identified PTKs showed highest expression during the estrous cycle and were down-regulated during pregnancy and lactation. Deregulated expression was rarely observed in the non-invasive mammary tumors. In contrast, 10 of 19 PTKs expressed during the estrous cycle were also over-expressed in the invasive carcinomas, mostly involving members of the receptor family of PTKs. |
| Databáze: | OpenAIRE |
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