Newcastle disease virus vectored rabies vaccine induces strong humoral and cell mediated immune responses in mice
| Autor: | Narayan Ramamurthy, Rahul Batheja, Vikram N. Vakharia, Sohini Dey, Madhan Mohan Chellappa, Ashis Debnath, Dinesh C. Pathak, Ajai Lawrence D’Silva |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Rabies
Genetic Vectors Newcastle disease virus medicine.disease_cause Kidney Vaccines Attenuated Microbiology Newcastle disease Virus Cell Line 03 medical and health sciences Mice Rabies vaccine Immune system Cricetinae Chlorocebus aethiops medicine Animals Vero Cells 030304 developmental biology Ovum 0303 health sciences Immunity Cellular Vaccines Synthetic General Veterinary biology 030306 microbiology Rabies virus General Medicine medicine.disease Vaccine efficacy biology.organism_classification Virology Fusion protein Immunity Humoral Specific Pathogen-Free Organisms Rabies Vaccines Chickens medicine.drug |
| Zdroj: | Veterinary microbiology. 251 |
| ISSN: | 1873-2542 |
| Popis: | Rabies is a devastating disease affecting almost all mammalian animal species including humans. Vaccines are available to combat the disease. Protection against the disease is rendered by assessing the humoral immune response. Recent reports suggest the role of cell mediated immune response (CMI) in assessing vaccine efficacy. In the present study, two live vectored vaccine candidates containing glycoprotein G of rabies virus were generated using the mesogenic Newcastle disease virus (NDV) strain R2B and another with NDV with an altered fusion protein cleavage site as backbones. The efficacy of these vaccine candidates on testing in experimental mouse model indicated generation of robust humoral and CMI responses. The recombinant NDV containing the altered fusion protein cleavage site with glycoprotein G showed the highest CMI response in mice indicating its usage as a potential live vectored vaccine candidate against the disease. |
| Databáze: | OpenAIRE |
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