Comparison of Sputum and Nasopharyngeal Aspirate Samples and of the PCR Gene Targets lytA and Spn9802 for Quantitative PCR for Rapid Detection of Pneumococcal Pneumonia
| Autor: | Per Olcén, Björn Herrmann, Kristoffer Strålin, Paula Mölling, Hans Holmberg, Guma Abdeldaim |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Adult Male Adolescent Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Sensitivity and Specificity Microbiology Young Adult Bacterial Proteins Nasopharyngeal aspirate Nasopharynx Streptococcus pneumoniae medicine Humans Aged Aged 80 and over Sputum Bacteriology Middle Aged Pneumonia Pneumococcal medicine.disease Penicillin Community-Acquired Infections Pneumonia Real-time polymerase chain reaction Molecular Diagnostic Techniques Pneumococcal pneumonia Etiology Female medicine.symptom medicine.drug |
| Popis: | We aimed to compare sputum and nasopharyngeal aspirate (NpA) samples and the PCR gene targets lytA and Spn9802 in quantitative PCR (qPCR) assays for rapid detection of pneumococcal etiology in community-acquired pneumonia (CAP). Seventy-eight adult patients hospitalized for radiologically confirmed CAP had both good-quality sputum and NpA specimens collected at admission. These samples were subjected to lytA qPCR and Spn9802 qPCR assays with analytical times of lytA assay), 96% and 17% (sputum Spn9802 assay), 81% and 11% (NpA lytA assay), and 81% and 20% (NpA Spn9802 assay), respectively. The mean lytA and Spn9802 DNA levels were significantly higher in qPCR-positive sputum samples from cases with pneumococcal etiology than in qPCR-positive sputum samples from CAP cases without pneumococcal etiology or qPCR-positive NpA samples from cases with pneumococcal etiology ( P < 0.02 for all comparisons). For detection of pneumococcal etiology, receiver operating characteristic curve analysis showed that sputum specimens were superior to NpA specimens as the sample type ( P < 0.02 for both gene targets) and lytA tended to be superior to Spn9802 as the gene target. The best-performing test, the sputum lytA qPCR assay, showed high sensitivity (94%) and specificity (96%) with a cutoff value of 10 5 DNA copies/ml. In CAP patients with good sputum production, this test has great potential to be used for the rapid detection of pneumococcal etiology and to target penicillin therapy. |
| Databáze: | OpenAIRE |
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