Application of quinpirole in the paraventricular thalamus facilitates emergence from isoflurane anesthesia in mice
| Autor: | Bo Yang, Caiju Zhang, Haibo Xu, Yawen Ao, Sirui Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Agonist
Quinpirole medicine.drug_class Neurosciences. Biological psychiatry. Neuropsychiatry 050105 experimental psychology 03 medical and health sciences Behavioral Neuroscience Mice 0302 clinical medicine Thalamus Dopamine mental disorders medicine emergence Animals 0501 psychology and cognitive sciences Anesthesia Original Research Raclopride isoflurane anesthesia Isoflurane business.industry 05 social sciences Dopaminergic Antagonist electroencephalogram paraventricular thalamus Dopamine Agonists Wakefulness business 030217 neurology & neurosurgery psychological phenomena and processes RC321-571 medicine.drug |
| Zdroj: | Brain and Behavior Brain and Behavior, Vol 11, Iss 1, Pp n/a-n/a (2021) |
| ISSN: | 2162-3279 |
| Popis: | Background and Purpose Dopamine is well‐known to contribute to emergence from anesthesia. Previous studies have demonstrated that the paraventricular thalamus (PVT) in the midline nuclei is crucial for wakefulness. Moreover, the PVT receives dopaminergic projections from the brainstem. Therefore, we hypothesize that the dopaminergic signaling in the PVT plays a role in emergence from isoflurane anesthesia. Methods We used c‐Fos immunohistochemistry to reveal the activity of PVT neurons in three groups: The first group (iso+EM‐) underwent the anesthesia protocol and was sacrificed before emergence. The second group (iso+EM+) underwent passive emergence from the same anesthesia protocol. The last group (oxy+) received oxygen. D2‐like agonist quinpirole (2 or 4 mM) or D2‐like antagonist raclopride (2 or 5 mM) was microinjected into the PVT, and their effects on emergence and induction time were analyzed. Surface cortical electroencephalogram (EEG) recordings were used to explore the effects of quinpirole injection into the PVT on cortical excitability during isoflurane anesthesia. The activity of PVT neurons after quinpirole injection was assessed by c‐Fos immunohistochemistry. Results The number of c‐Fos‐positive nuclei for the iso+EM+ group was significantly higher than the oxy+ and iso+EM‐ groups. Application of quinpirole (4 mM) into the PVT shortened emergence time compared with the saline group (p The activity of PVT neurons is enhanced after emergence from isoflurane anesthesia in mice. The dopaminergic signaling in the PVT facilitates emergence from isoflurane anesthesia. |
| Databáze: | OpenAIRE |
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