Two different activities of alkaline phosphatase in cultured mammalian cells
| Autor: | Hajim Katsuta, Toshiko Takaoka, Kiyoshi Nose |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Carcinoma
Hepatocellular Cells Cyanide Acid Phosphatase Cell Biophysics Thymus Gland Biology Biochemistry Chromatography DEAE-Cellulose Mice chemistry.chemical_compound Drug Stability Mammalian cell medicine Animals Molecular Biology Cells Cultured Strain (chemistry) Liver Neoplasms Temperature Acid phosphatase Fibroblasts Hydrogen-Ion Concentration Alkaline Phosphatase Molecular biology Rats medicine.anatomical_structure Liver chemistry biology.protein Alkaline phosphatase Subcellular Fractions |
| Zdroj: | Archives of Biochemistry and Biophysics. 155:1-8 |
| ISSN: | 0003-9861 |
| Popis: | Alkaline phosphatase activities in various mammalian cell strains in culture, some of which had been grown in protein-free synthetic media, were investigated and characterized. Two distinctly different activities of alkaline phosphatase were found in crude extracts of various cell strains. The first activity (designated alkaline phosphatase I) was the highest around the range of pH 10.0 and inhibited by cyanide or β -mercapto-ethanol. This activity was mainly detected in particulate fractions of cells. The second was shown to be optimal at pH 8.6 and inhibited by p -chloromercuribenzoate (designated alkaline phosphatase II). Among various cell strains examined, the former activity was detected only in some of them, whereas the latter type was present in each strain. These two activities were separated from each other by DEAE-cellulose chromatography. |
| Databáze: | OpenAIRE |
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