MicroRNA‐155 is a novel suppressor of ovarian cancer‐initiating cells that targets CLDN1
| Autor: | Jiejun Wang, Qiusheng Ren, Wenxing Qin, Te Liu, Yongyi Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cell
Biophysics Down-Regulation Biology medicine.disease_cause Biochemistry Mice Dogs Invasion Downregulation and upregulation Cell Movement Structural Biology Cell Line Tumor Claudin-1 microRNA Genetics medicine Animals Humans Neoplasm Invasiveness Luciferase 3' Untranslated Regions Molecular Biology Cell Proliferation Ovarian Neoplasms Regulation of gene expression Mice Inbred BALB C Binding Sites Base Sequence Cell growth Cell Biology medicine.disease Gene Expression Regulation Neoplastic MicroRNAs Proto-Oncogene Proteins c-kit Cell Transformation Neoplastic Hyaluronan Receptors medicine.anatomical_structure CLDN1 Cancer research Female Carcinogenesis Ovarian cancer Ovarian cancer-initiating cell (OCIC) MicroRNA-155 Cell Adhesion Molecules |
| Zdroj: | FEBS Letters. 587:1434-1439 |
| ISSN: | 1873-3468 0014-5793 |
| Popis: | Previous cDNA microarrays indicated that CLDN1 (claudin-1) is an important gene for ovarian cancer-initiating cell (OCIC) invasion and adhesion. Here, we show that the downregulation of miR-155 in OCICs correlates with CLDN1 overexpression and the suppression of OCIC invasion. Luciferase assays indicate that miR-155 targets CLDN1 mRNA on the 3′ UTR. CLDN1 mRNA and claudin-1 protein expression were significantly decreased in miR-155-OCICs. Proliferation assays and Transwell migration assays show that miR-155 significantly suppresses the proliferative and invasive capacity of OCICs. Furthermore, miR-155 suppresses the growth of OCIC xenograft tumors. Thus, overexpression of miR-155 may prevent tumorigenesis in human ovarian cancer through downregulation of CLDN1. |
| Databáze: | OpenAIRE |
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