The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function
| Autor: | Signe Spetalen, Tor Lea, Odd Helge Romarheim, Charlotte R. Kleiveland, Oskar Bengtsson, Trine Eker Christofferesen, Magne Kaldhusdal, Lene T. Olsen Hult, Morten Jacobsen |
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| Rok vydání: | 2013 |
| Předmět: |
Colon
Ralstonia Mucin 2 Applied Microbiology and Biotechnology Inflammatory bowel disease Microbiology Epithelial Damage Mice Intestinal mucosa medicine Animals Intestinal Mucosa Colitis Methylococcus capsulatus Barrier function Bacillales Mucin-2 Ecology biology Dextran Sulfate biology.organism_classification medicine.disease Ulcerative colitis Diet Mice Inbred C57BL Disease Models Animal Food Microbiology Colitis Ulcerative Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 79:48-56 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.02464-12 |
| Popis: | Dietary inclusion of a bacterial meal has recently been shown to efficiently abolish soybean meal-induced enteritis in Atlantic salmon. The objective of this study was to investigate whether inclusion of this bacterial meal in the diet could abrogate disease development in a murine model of epithelial injury and colitis and thus possibly have therapeutic potential in human inflammatory bowel disease. C57BL/6N mice were fed ad libitum a control diet or an experimental diet containing 254 g/kg of body weight BioProtein, a bacterial meal consisting of Methylococcus capsulatus (Bath), together with the heterogenic bacteria Ralstonia sp., Brevibacillus agri , and Aneurinibacillus sp. At day 8, colitis was induced by 3.5% dextran sulfate sodium (DSS) ad libitum in the drinking water for 6 days. Symptoms of DSS treatment were less profound after prophylactic treatment with the diet containing the BioProtein. Colitis-associated parameters such as reduced body weight, colon shortening, and epithelial damage also showed significant improvement. Levels of acute-phase reactants, proteins whose plasma concentrations increase in response to inflammation, and neutrophil infiltration were reduced. On the other, increased epithelial cell proliferation and enhanced mucin 2 (Muc2) transcription indicated improved integrity of the colonic epithelial layer. BioProtein mainly consists of Methylococcus capsulatus (Bath) (88%). The results that we obtained when using a bacterial meal consisting of M. capsulatus (Bath) were similar to those obtained when using BioProtein in the DSS model. Our results show that a bacterial meal of the noncommensal bacterium M. capsulatus (Bath) has the potential to attenuate DSS-induced colitis in mice by enhancing colonic barrier function, as judged by increased epithelial proliferation and increased Muc2 transcription. |
| Databáze: | OpenAIRE |
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