Biomimetic apatite-coated porous PVA scaffolds promote the growth of breast cancer cells
| Autor: | Gargi Ghosh, Pravansu S. Mohanty, Mao Ye |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Vinyl alcohol
Materials science Scanning electron microscope Nucleation Breast Neoplasms Bioengineering Nanotechnology Balanced salt solution Bone tissue Mineralization (biology) Apatite Biomaterials chemistry.chemical_compound Adsorption Biomimetic Materials Cell Line Tumor Cell Adhesion medicine Humans Polyvinyl Chloride Cell Proliferation Tissue Scaffolds Durapatite Freeze Drying medicine.anatomical_structure chemistry Chemical engineering Mechanics of Materials visual_art Microscopy Electron Scanning visual_art.visual_art_medium Female Porosity |
| Zdroj: | Materials Science and Engineering: C. 44:310-316 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2014.08.044 |
| Popis: | Recapitulating the native environment of bone tissue is essential to develop in vitro models of breast cancer bone metastasis. The bone is a composite material consisting of organic matrix and inorganic mineral phase, primarily hydroxyapatite. In this study, we report the mineralization of porous poly vinyl alcohol (PVA) scaffolds upon incubation in modified Hanks' Balanced Salt Solution (HBSS) for 14 days. Scanning electron microscopy, energy dispersive X-ray analysis, and X-ray diffraction analysis revealed that the deposited minerals have composition similar to hydroxyapatite. The study demonstrated that the rate of nucleation and growth of minerals was faster on surfaces of less porous scaffolds. However, upon prolonged incubation, formation of mineral layer was observed on the surface of all the scaffolds. In addition, the study also demonstrated that 3D mineralization only occurred for scaffolds with highly interconnected porous networks. The mineralization of the scaffolds promoted the adsorption of serum proteins and consequently, the adhesion and proliferation of breast cancer cells. |
| Databáze: | OpenAIRE |
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