Expansion of Gamma-Butyrolactone Signaling Molecule Biosynthesis to Phosphotriester Natural Products
| Autor: | Bradley S. Moore, Kaitlin E. Creamer, Yi-Ling Du, Roger G. Linington, Katherine S. Ryan, Takayoshi Awakawa, Paul R. Jensen, Yuta Kudo, Peter A. Jordan |
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| Rok vydání: | 2020 |
| Předmět: |
Biological Products
Cell signaling biology Operon Chemistry Esters General Medicine Bridged Bicyclo Compounds Heterocyclic biology.organism_classification Biochemistry Streptomyces Article In vitro chemistry.chemical_compound 4-Butyrolactone Biosynthesis Genes Bacterial Gene cluster Molecular Medicine Gene Bacteria Signal Transduction |
| Zdroj: | ACS Chem Biol |
| ISSN: | 1554-8937 1554-8929 |
| DOI: | 10.1021/acschembio.0c00824 |
| Popis: | Bacterial hormones, such as the iconic gamma-butyrolactone A-factor, are essential signaling molecules that regulate diverse physiological processes, including specialized metabolism. These low molecular weight compounds are common in Streptomyces species and display species–specific structural differences. Recently, unusual gamma-butyrolactone natural products called salinipostins were isolated from the marine actinomycete genus Salinispora based on their antimalarial properties. As the salinipostins possess a rare phosphotriester motif of unknown biosynthetic origin, we set out to explore its construction by the widely conserved 9-gene spt operon in Salinispora species. We show through a series of in vivo and in vitro studies that the spt gene cluster dually encodes the salinipostins and newly identified natural A-factor-like gamma-butyrolactones (Sal-GBLs). Remarkably, homologous biosynthetic gene clusters are widely distributed among many actinomycete genera, including Streptomyces, suggesting the significance of this operon in bacteria. |
| Databáze: | OpenAIRE |
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