Myogenic expression of an injectable protease-resistant growth hormone–releasing hormone augments long-term growth in pigs
| Autor: | Robert J. Schwartz, L. A. Hill, P. B. Malone, Ruxandra Draghia-Akli, D. R. Deaver, Marta L. Fiorotto |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Swine medicine.medical_treatment Biomedical Engineering Bioengineering Biology Growth Hormone-Releasing Hormone Muscle Development Weight Gain Injections Intramuscular Applied Microbiology and Biotechnology Internal medicine Endopeptidases medicine Animals Insulin-Like Growth Factor I Muscle Skeletal Cells Cultured Protease Catabolism Growth factor Genetic transfer Gene Transfer Techniques Growth hormone–releasing hormone Growth hormone secretion Endocrinology Gene Expression Regulation Growth Hormone Molecular Medicine Ectopic expression Plasmids Biotechnology Hormone |
| Zdroj: | Nature Biotechnology. 17:1179-1183 |
| ISSN: | 1546-1696 1087-0156 |
| Popis: | Ectopic expression of a new serum protease-resistant porcine growth hormone-releasing hormone, directed by an injectable muscle-specific synthetic promoter plasmid vector (pSP-HV-GHRH), elicits growth in pigs. A single 10 mg intramuscular injection of pSP-HV-GHRH DNA followed by electroporation in three-week-old piglets elevated serum GHRH levels by twofold to fourfold, enhanced growth hormone secretion, and increased serum insulin-like growth factor-I by threefold to sixfold over control pigs. After 65 days the average body weight of the pigs injected with pSP-HV-GHRH was approximately 37% greater than the placebo-injected controls and resulted in a significant reduction in serum urea concentration, indicating a decrease in amino acid catabolism. Evaluation of body composition indicated a uniform increase in mass, with no organomegaly or associated pathology. |
| Databáze: | OpenAIRE |
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