Simultaneous assay of adenosine deaminase and purine nucleoside phosphorylase activity as possible biochemical means to detect non‐hodgkin lymphomas of the oral cavity
| Autor: | Takao Shibata, Akio Mizuno, Kazuhiko Morita, Yasuhisa Naito, Keizo Maeda |
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| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
Adenosine Deaminase Gingiva Purine nucleoside phosphorylase Adenosine deaminase immune system diseases Biopsy Humans Medicine Lymphocytes Skin Inflammation chemistry.chemical_classification Osteoblasts biology medicine.diagnostic_test business.industry Lymphoma Non-Hodgkin Mouth Mucosa Clinical Enzyme Tests Fibroblasts Hyperplasia medicine.disease Gingivitis Molecular biology In vitro Squamous carcinoma enzymes and coenzymes (carbohydrates) Enzyme Purine-nucleoside phosphorylase activity Purine-Nucleoside Phosphorylase Oncology chemistry Biochemistry biology.protein Mouth Neoplasms Lymph Nodes business Granulocytes |
| Zdroj: | Cancer. 70:20-27 |
| ISSN: | 1097-0142 0008-543X |
| Popis: | Background and Methods. This study proposes the usefulness of simultaneous assay of adenosine deaminase (enzyme code [EC] 3.5.4.4.; ADA) and purine nucleoside phosphorylase (EC 2.4.2.1.; PNP) activities in a biopsy specimen as a biochemical test adjunctive to the histologic diagnosis of oral non-Hodgkin lymphomas (NHL). Results. NHL tissues showed an ADA activity of more than 1428 nmol/h/mg of protein, and more than 3451 nmol/h/mg of protein was the sum of ADA and PNP activity. In contrast to this finding, all the 106 lesional tissues affected by various carcinomas, various benign tumors, various cysts, ameloblastomas, osteosarcomas, gingival hyperplasia, and inflammation showed an ADA activity of less than 1000 nmol/h/mg of protein, except for 2. The sum of ADA and PNP activity was less than 2249 nmol/h/mg of protein in these diseases, except for squamous carcinoma. Different control tissues of the oral cavity showed less than 766 nmol/h/mg of protein in ADA activity, and the highest sum of ADA and PNP activity in these was only 1384 nmol/h/mg of protein. Conclusions. These findings suggest that a strong suspicion of oral NHL is justified when the sum of ADA and PNP activity in a biopsy specimen exceeds 3000 nmol/h/mg of protein and ADA activity is greater than 1000 nmol/h/mg of protein. |
| Databáze: | OpenAIRE |
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