Poly (A) RNA inBacillus subtilis: Identification of the polyadenylylation site of flagellin mRNA
| Autor: | Nilima Sarkar, Gong-jie Cao |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Polyadenylation
Molecular Sequence Data Bacillus subtilis Gram-Positive Bacteria Primary transcript Microbiology chemistry.chemical_compound Sequence Homology Nucleic Acid Gram-Negative Bacteria Escherichia coli Genetics Amino Acid Sequence RNA Messenger RNA Processing Post-Transcriptional Molecular Biology Gene Terminator Regions Genetic Messenger RNA Base Sequence biology RNA DNA biology.organism_classification Molecular biology RNA Bacterial chemistry biology.protein Nucleic Acid Conformation Poly A Flagellin |
| Zdroj: | FEMS Microbiology Letters. 108:281-285 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1993.tb06116.x |
| Popis: | Using improved procedures for the synthesis, amplification, and cloning of DNA complementary to bacterial poly(A) RNA, we succeeded in deducing the 3′-terminal sequence of polyadenylylated flagellin mRNA encoded by the Bacillus subtilis hag gene. The site of polyadenylylation was found to be just upstream of the terminal stem-loop structure corresponding to the putative rho-independent transcription terminator of the hag gene. This corresponds to the major polyadenylation site of Escherichia coli lipoprotein ( lpp ) mRNA [G.C. Gao and N. Sarkar (1992) Proc. Natl. Acad. Sci. USA 89, 7546–7550], suggesting that the primary transcript is processed by endonucleolytic cleavage prior to polyadenylylation in Gram-positive as well as in Gram-negative bacteria. |
| Databáze: | OpenAIRE |
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