Kinetics of mature T-cell development in the thymus
| Autor: | Mark Egerton, Ken Shortman, Roland Scollay |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte DNA Replication Male CD3 Complex Cell division CD8 Antigens T-Lymphocytes CD3 Cellular differentiation Receptors Antigen T-Cell Thymus Gland Biology Tritium Mice Animals Multidisciplinary T lymphocyte Cell sorting Cell biology Kinetics Thymocyte Mature Thymocyte Biochemistry CD4 Antigens Mice Inbred CBA biology.protein Cell Division CD8 Research Article Thymidine |
| Zdroj: | Proceedings of the National Academy of Sciences. 87:2579-2582 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.87.7.2579 |
| Popis: | We have reexamined the balance between cell birth, cell maturation, and cell death in the thymus by labeling dividing thymocytes and their progeny in vivo with [3H]-thymidine, isolating clearly defined subpopulations by fluorescence-activated cell sorting, and determining the distribution of label by autoradiography. When mature thymocytes were precisely defined (as CD4+CD8- CD3+ or CD4-CD8+ CD3+) and separated from immature single positives (CD4+CD8- CD3- and CD4-CD8+ CD3-), a lag was observed in the rate of entry of [3H]thymidine into mature cells. Thus, many of the mature thymocytes appear to derive from a small nondividing cortical thymocyte pool, rather than originating directly from the earliest dividing CD4+CD8+ blasts. There was little evidence for cell division during or after mature thymocyte formation, suggesting a one-for-one differentiation from cortical cells rather than selective clonal expansion. The rate of production of mature single positive thymocytes agreed closely with estimates of the rate of export of mature T cells from the thymus and was only 3% of the rate of production of double-positive cortical thymocytes. This was compatible with a stringent selection process and extensive intrathymic cell death and suggested that no extensive negative selection occurred after the mature cells were formed. |
| Databáze: | OpenAIRE |
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