Directional Growth of Human Neuronal Axons in a Microfluidic Device with Nanotopography on Azobenzene-Based Material
| Autor: | Susanna Narkilahti, Ropafadzo Mzezewa, Sanna Hagman, Lassi Sukki, Arri Priimagi, Fikret E. Kapucu, Pasi Kallio, Mervi Ristola, Tanja Hyvärinen, Chiara Fedele |
|---|---|
| Přispěvatelé: | Tampere University, Clinical Medicine, Materials Science and Environmental Engineering, BioMediTech |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Thesaurus (information retrieval) Materials science 318 Medical biotechnology Mechanical Engineering Microfluidics Nanotechnology 02 engineering and technology 217 Medical engineering 021001 nanoscience & nanotechnology 03 medical and health sciences chemistry.chemical_compound Azobenzene chemistry nervous system Mechanics of Materials 216 Materials engineering Nanotopography 0210 nano-technology 030304 developmental biology |
| Popis: | Axonal dysfunction and degeneration are important pathological features of central nervous system (CNS) diseases and traumas, such as Alzheimer's disease, traumatic brain injury, ischemic stroke and spinal cord injury. Engineered microfluidic chips combined with human pluripotent stem cell (hPSC)-derived neurons provide valuable tools for targeted in vitro research on axons to improve understanding of disease mechanisms and enhance drug development. Here, a polydimethylsiloxane (PDMS) microfluidic chip integrated with a light patterned substrate is utilized to achieve both isolated and unidirectional axonal growth of hPSC-derived neurons. The isolation of axons from somas and dendrites and robust axonal outgrowth to adjacent, axonal compartment, is achieved by optimized cross-sectional area and length of PDMS microtunnels in the microfluidic device. In the axonal compartment, the photoinscribed nanotopography on a thin film of azobenzene-containing molecular glass efficiently guides the growth of axons. Integration of nanotopographic patterns with a compartmentalized microfluidic chip creates a human neuron-based model that supports superior axonal alignment in an isolated microenvironment. The practical utility of the chip by studying oxygen-glucose deprivation-induced damage for the isolated and aligned axons is demonstrated here. The created chip model represents a sophisticated platform and a novel tool for enhanced and long-term axon-targeted in vitro studies. publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|