Relaxation of sheep cerebral arteries by vasoactive intestinal polypeptide and neurogenic stimulation: inhibition by L-NG-monomethyl arginine in endothelium-denuded vessels
| Autor: | Geoffrey Burnstock, Roger M. Wadsworth, Alasdair J. Gaw, Patrick P.A. Humphrey, J. Aberdeen |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Endothelium Arginine Calcitonin Gene-Related Peptide Vasoactive intestinal peptide Cerebral arteries Vasodilation Calcitonin gene-related peptide In Vitro Techniques Nitric Oxide Internal medicine medicine.artery Medicine Animals Pharmacology Sheep omega-N-Methylarginine business.industry Cerebral Arteries Immunohistochemistry Electric Stimulation Endocrinology medicine.anatomical_structure Middle cerebral artery Omega-N-Methylarginine Endothelium Vascular business Research Article Vasoactive Intestinal Peptide |
| Popis: | 1. Perivascular nerves of the sheep middle cerebral artery show immunoreactivity for both vasoactive intestinal polypeptide (VIP) and calcitonin gene-related peptide (CGRP). 2. Rings of endothelium-denuded sheep middle cerebral artery precontracted with 5-hydroxytryptamine were relaxed by CGRP (maximum relaxation = 87.8 +/- 8.1%, pD2 = 7.81 +/- 0.12, n = 12) and by VIP (maximum relaxation = 55.1 +/- 4.1%, pD2 = 7.65 +/- 0.04, n = 18). Rings of endothelium-denuded cat middle cerebral artery precontracted with U46619 were also relaxed by vasoactive intestinal polypeptide (maximum relaxation = 53.1 +/- 6.1%, pD2 = 7.82 +/- 0.11, n = 6). 3. Haemolysate (1 microliters ml-1) inhibited VIP-induced relaxation in endothelium-denuded sheep and cat middle cerebral artery (n = 6) but had no effect on the CGRP-induced relaxation of the sheep middle cerebral artery (n = 6). 4. The relaxant response to VIP in endothelium-denuded sheep middle cerebral artery was inhibited by methylene blue (10 microM) and augmented by either M&B 22948 (10 microM) or superoxide dismutase (150 units ml-1). Indomethacin (1 microM) had no effect. 5. The addition of L-NG-monomethyl arginine (100 microM) inhibited both neurogenic and VIP-induced relaxation of endothelium-denuded sheep MCA by 56 +/- 6% and 60 +/- 6% (n = 5) respectively. The CGRP-induced relaxation was unaffected. 6. It is concluded that neurally mediated vasodilatation in the sheep middle cerebral artery is mediated largely by VIP through a direct action on smooth muscle through a cyclic-GMP-mediated mechanism that appears to involve synthesis of nitric oxide from L-arginine. Vasodilatation by CGRP, which is also contained in perivascular nerves, does not utilize this pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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