Purification of toxic saponins from Narthecium asiaticum Maxim
| Autor: | Kazue Suzuki, Masato Kobayashi, Shigeyoshi Nagasawa, Yoshihiro Mimaki |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Arabinose
Diarrhea Magnetic Resonance Spectroscopy Guinea Pigs Saponin Mass Spectrometry chemistry.chemical_compound Column chromatography Enzymatic hydrolysis Animals Trisaccharide chemistry.chemical_classification Chromatography General Veterinary Molecular Structure Sarsasapogenin Saponins Anorexia Plants Toxic chemistry Biochemistry Narthecium asiaticum Galactose Chromatography Thin Layer Gastrointestinal Hemorrhage Trisaccharides |
| Zdroj: | The Journal of veterinary medical science. 55(3) |
| ISSN: | 0916-7250 |
| Popis: | Toxic substances in Narthecium asiaticum Maxim. were isolated and purified, monitoring the oral toxicity in guinea pigs. The crude extract prepared from the methanolic extract of the plant contained 2 major saponins (C8 and C9), and 7 corresponding saponins (C1-7) on TLC. The crude extract was fractionated by a conventional method for saponin preparation. The 1-butanol fraction was rich in C8 and C9, and showed oral toxicity. C8 and C9 were isolated by fractional precipitation and silica gel column chromatography. On the basis of C-13 and H-1 nuclear magnetic resonance (NMR) spectral data, and acid and enzymatic hydrolysis of C9, C9 was confirmed to be a mixture of two furostanol saponins (C9a, C9b); a branched trisaccharide composed of galactose, glucose, and arabinose, was linked at 3 beta-C, and glucose at 26-C of sarsasapogenin and smilagenin. C8 was considered to be a furostanol saponin containing xylose instead of arabinose in the structure of C9. Oral administration of C8 and C9 caused diarrhea, proteinuria, hematuria and death in guinea pigs. |
| Databáze: | OpenAIRE |
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