In vitro histomorphometric comparison of dental pulp tissue in different teeth
| Autor: | Marytere Guerrero-Jiménez, Nelly C Castro-Linares, Fernando Javier Aguilar-Ayala, Michel Canul-Chan, Rafael Rojas-Herrera, Geovanny I. Nic-Can, Beatriz A. Rodas-Junco, Ricardo Peñaloza-Cuevas |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Molar
lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine stomatognathic system Dental pulp stem cells Premolar medicine Deciduous teeth Supernumerary Maxillary central incisor 030304 developmental biology Permanent teeth Orthodontics 0303 health sciences Stem cell General Neuroscience lcsh:R 030206 dentistry General Medicine Cell Biology Dental pulp stomatognathic diseases medicine.anatomical_structure Histomorphometric Dentistry Pulp (tooth) General Agricultural and Biological Sciences Supernumerary teeth |
| Zdroj: | PeerJ, Vol 7, p e8212 (2019) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | Background Dental pulp (DP) represents an accessible and valuable source promising of stem cells for clinical application. However, there are some disadvantages associated with the isolation of dental pulp stem cells (DPSCs), which include the size and weight of the pulp tissue needed to yield sufficient cells for culturing in vitro. Therefore, the objective of this study was to compare in vitro histomorphometry of DP from permanent (premolars, third molar), supernumerary and deciduous teeth of patients between 5 and 25 years old with regards to weight, length, width and the cell density in the four regions of the DP in order to obtain quantitative parameters in a tissue that represents a valuable source of stem cells. Methods DPs were obtained from 10 central incisors deciduous, 20 permanent teeth (10 premolars, 10 third molars) and 10 supernumeraries (six mesiodents and four inferior premolar shapes). The pulps were carefully removed, and the entire tissue was weighed. The pulp length and the width were measured with a digital Vernier caliper. The cellular density analysis was performed according to the four regions of the DP (coronal, cervical, medial and apical) in histological slides using photography and the ImageJ® program for quantification. Results The Pearson correlation test revealed that DP weight among different types of teeth is correlated with age in male patients. A significant positive correlation was noted between length and width of the DP with age in both genders. The mean DP weight for supernumerary and third molar teeth was greater than deciduous and premolar teeth. Finally, the histological analysis showed that the coronal and apical portions of DP in supernumerary and premolar teeth have the highest cell density. Conclusions The DP of supernumerary teeth has quantitatively the best morphometric parameters and cell density comparable with the quality of DP obtained from deciduous teeth. |
| Databáze: | OpenAIRE |
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