Molecular karyotype analysis of Perkinsus atlanticus (Phylum Perkinsozoa) by pulsed field gel electrophoresis
| Autor: | Anabela Cordeiro-da-Silva, Alexandre Gaspar-Maia, António Rocha, Joana Tato-Costa, Carlos Azevedo, Américo Marques, M. Leonor Teles-Grilo, Carla Oliveira, Sergio Duarte |
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| Rok vydání: | 2007 |
| Předmět: |
biology
Ecology Phylum Lineage (evolution) Dinoflagellate Protist Eukaryota Karyotype DNA Protozoan biology.organism_classification medicine.disease_cause Microbiology Electrophoresis Gel Pulsed-Field Evolutionary biology Genus Karyotyping Pulsed-field gel electrophoresis medicine Animals Perkinsus Densitometry |
| Zdroj: | European journal of protistology. 43(4) |
| ISSN: | 0932-4739 |
| Popis: | Perkinsus atlanticus is a pathogenic protist that infects the clam Ruditapes decussatus. Although it was recently proposed that the genus Perkinsus belongs to a new phylum, Perkinsozoa, in the infra-kingdom Alveolata, there remain different opinions about whether this genus should form a phylum on its own and consequently divergent views about its taxonomic characterization. In this work, we have identified nine chromosomes by pulsed field gel electrophoresis (PFGE) combined with densitometry analysis. The obtained karyotype of Perkinsus atlanticus, like that of other early branches of the dinoflagellate lineage, displays a more conventional chromosome organization, different from that of most dinoflagellates. |
| Databáze: | OpenAIRE |
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