Typeability of AmpFlSTR SGM Plus Loci in Brain and Thyroid Gland Tissue Samples Incubated in Different Environments
| Autor: | Jerzy Janica J, Małgorzata Skawrońska, Jacek Janica, Ewa Koc-Zorawska, Anna Niemcunowicz-Janica, Witold Pepinski |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Forensic Genetics Male Thyroid Gland Brain tissue Biology Polymerase Chain Reaction Pathology and Forensic Medicine DNA degradation Genetics medicine Humans Putrefaction Genotyping Brain Chemistry Thyroid Brain DNA Thyroid Gland Tissue DNA Fingerprinting Molecular biology Environmental effect medicine.anatomical_structure Postmortem Changes Female Amelogenin |
| Zdroj: | Journal of Forensic Sciences. 52:867-869 |
| ISSN: | 1556-4029 0022-1198 |
| Popis: | Autolysis and putrefaction are crucial factors responsible for degradation of cells, tissues, and organs. Postmortem changes may assume different course depending on extrinsic and intrinsic conditions. The aim of the study was assessment of environmental effect on typeability of AmpFlSTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA, and gender marker amelogenin. Brain and thyroid gland tissue specimens collected during autopsies of five persons aged 20-30 years were incubated at 21 degrees C and 4 degrees C in different environmental conditions. DNA was extracted by organic method from tissue samples collected in 7-day intervals and subsequently typed using AmpFlSTR SGM Plus kit and ABI 310. A fast decrease in typeability rate was seen in specimens incubated in peat soil and in sand. Brain tissue samples were typeable in all AmpFlSTR SGM Plus loci within 126 days of incubation at 4 degrees C. Faster DNA degradation was recorded in thyroid gland specimens. In samples with negative genotyping results, no DNA was found by fluorometric quantitiation. |
| Databáze: | OpenAIRE |
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