Genetic polymorphism of plasma α1B-glycoprotein and transferrin in arctic and silver foxes
| Autor: | R. K. Juneja, B. Larsen, B. Gahne, T. Niini, O. Lohi |
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| Rok vydání: | 2009 |
| Předmět: |
Male
animal diseases Foxes Alpha-1-B glycoprotein Biology Species Specificity parasitic diseases Genetics Animals Allele Polyacrylamide gel electrophoresis Alleles Glycoproteins Gel electrophoresis chemistry.chemical_classification Polymorphism Genetic Transferrin food and beverages Blood Proteins General Medicine Molecular biology Blood proteins Staining Arctic chemistry Hybridization Genetic population characteristics Female Animal Science and Zoology geographic locations |
| Zdroj: | Animal Genetics. 19:237-244 |
| ISSN: | 1365-2052 0268-9146 |
| Popis: | Plasma samples of 235 foxes from 38 complete families (14 of arctic foxes, 21 of silver foxes and 3 with arctic x silver fox hybrid offspring) were analysed by one-dimensional horizontal polyacrylamide gel electrophoresis (PAGE) pH 9.0 followed by general-protein staining of gels. A major postalbumin of fox plasma was identified as alpha 1B-glycoprotein (alpha 1B) by using immunoblotting with antiser m specific to human or pig plasma alpha 1B. Four codominant, autosomal alleles of alpha 1B were found in arctic foxes. Two transferrin (TF) alleles (TfF, TfS) were observed in arctic foxes and two (TfD, Tff) in silver foxes; the TF F type of both of the fox species showed identical electrophoretic mobilities. The arctic foxes showed a high degree of polymorphism for both TF and alpha 1B. The silver foxes showed a scarce polymorphism of TF and were monomorphic for alpha 1B. The arctic fox, silver fox and their hybrids could be clearly differentiated from one another by their plasma protein patterns obtained by the PAGE method. |
| Databáze: | OpenAIRE |
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